Fig. 1.

IL-10 effectively upregulates osteoactivin expression in human moDC. Immature moDC were generated in vitro in the presence or absence (“none”) of IL-10 and analyzed for osteoactivin expression. Representative results from at least three independent experiments using different donors are presented. a qPCR analysis: relative level of osteoactivin mRNA. The mean (±SD) of duplicate measurements is shown. Osteoactivin protein level was analyzed by b western blotting and c flow cytometry. Open histograms represent staining with osteoactivin antibody; shaded histograms represent the isotype controls