Fig. 4.

TKI effectively upregulate osteoactivin expression in moDC. Immature moDC were generated in vitro in the presence or absence (“none”) of imatinib, nilotinib, or dasatinib and analyzed for osteoactivin expression. Representative results from at least three independent experiments using different donors are presented. a qPCR analysis: relative level of osteoactivin mRNA. The mean (±SD) of duplicate measurements is shown. Osteoactivin protein levels were analyzed by b western blotting and c flow cytometry. Open histograms represent staining with osteoactivin antibody; shaded histograms indicate isotype control staining