Fig. 5.

Detection of DDX3X in human cancer cells. a Whole cell lysates were extracted from 87.5 and S2 (human small cell lung cancer lines), HCT116 (a human colon cancer), A549 (a human, non-small cell lung cancer), WM115 (a human melanoma), and MCF7 (a human breast cancer) cells. Immunoblots from tumor cells were probed with antibodies against DDX3X and β-actin. b Human cancer cell lines, that is, 87.5 and S2 (small cell lung cancer lines), HCT116 (colon cancer cell line), A549 (non-small cell lung cancer cell line), WM115 (melanoma cell line), and MCF7 (breast cancer cell line) were examined for the expression of putative CSC markers. It was found that 87.5 and HCT116 cells expressed CD133, whereas the other cancer cells did not. MCF7 cells contained CD44⁺ and CD24⁻/low populations