Fig. 1.

Increasing TH1-cytokine expressions until day ± 200 in one representative patient (# 3). Figure 1 depicts representative results obtained by FACS analysis of PBMC from patient number # 3. Cells were stimulated with PMA and ionomycin, as described in the section of “Materials and methods”. Gating was performed on lymphocytes by scatter characteristics and CD3⁺ cells. The expression of IFNγ (upper lane) and TNFα (lower lane) in CD3-positive T-cells before (left column), 60 days (middle column) and 200 days (right column) post-transplant is shown