Table 3.
Immune reconstitution after autologous stem cell transplantation
| Mean values | Before autoHSCT | Engraftment | Day +30 | Day +60 | Day +100 | Day +365 | >2 years | Normal range |
|---|---|---|---|---|---|---|---|---|
| CD16/56/μl | 160 ± 125 | 100 ± 70 | 232 ± 250 | 197 ± 78 | 172 ± 75 | 152 ± 100 | 260 ± 73 | 300–600 |
| CD3/μl | 845 ± 498 | 354 ± 427 | 550 ± 253 | 456 ± 278 | 387 ± 142 | 837 ± 24 | 1,147 ± 335 | 1,800–3,600 |
| CD4/μl | 507 ± 352 | 175 ± 279 | 224 ± 183 | 249 ± 112 | 135 ± 90 | 422 ± 124 | 620 ± 156 | 800–1,800 |
| CD8/μl | 290 ± 140 | 89 ± 124 | 185 ± 81 | 224 ± 96 | 234 ± 112 | 322 ± 43 | 348 ± 43 | 800–1,500 |
| CD3CD45RA% | 52 ± 11 | 29 ± 16 | 28 ± 9 | 36 ± 8 | 36 ± 7 | 45 ± 8 | 44 ± 8 | 60–70 |
| CD3CD45RO% | 33 ± 12 | 53 ± 15 | 61 ± 10 | 53 ± 13 | 53 ± 7 | 43 ± 9 | 38 ± 8 | 21–40 |
| CD19/μl | 278 ± 194 | 62 ± 86 | 101 ± 124 | 146 ± 113 | 199 ± 129 | 148 ± 48 | 462 ± 144 | 500–1,300 |
| IgG mg/dl | 203 ± 64 | 193 ± 35 | 186 ± 25 | 197 ± 53 | 127 ± 88 | 200 ± 78 | 250 ± 134 | 570–1,500 |
| CD3+IL2% | 21 ± 9 | 50 ± 20 | 35 ± 9 | 35 ± 5 | 30 ± 4 | 20 ± 10 | 27 ± 9 | 33–52 |
| CD3+IFNγ% | 23 ± 6 | 21 ± 10 | 47 ± 13 | 52 ± 14 | 60 ± 5 | 60 ± 15 | 26 ± 5 | 15–30 |
| CD3+TNFα% | 23 ± 9 | 25 ± 5 | 38 ± 11 | 37 ± 12 | 55 ± 6 | 40 ± 15 | 31 ± 11 | 16–29 |
| CD3+IL4% | 0.5 ± 0.5 | 2 ± 0.5 | 0.5 ± 0.3 | 1 ± 0.5 | 1 ± 0.5 | 0.6 ± 0.4 | 1 ± 0 | 0.5–2 |
Lymphocyte reconstitution and cytokine expression in CD3+ T-cells before transplantation, at engraftment, on days +30, +100, +365 and >2 years post-transplantation. Cytokine production was determined by FACS after stimulation with PMA and ionomycin as described above. Percentages of cytokine-positive cells in CD3+ T-cells as mean ± SD are shown