Table 1.
Peptides derived from the HOM-MEL-40/SSX2 antigen selected for this study
| Sequence | Position | Binding probability to HLA-DRB1 | |||||
|---|---|---|---|---|---|---|---|
| *0101 | *0301 | *0401 | *0701 | *1101 | *1501 | ||
KIFYVYMKRKYEAMT |
p45-59 | 18 | 8 | 16 | 10 | 24 | 0 |
KLGFKATLPPFMCNK |
p60-74 | 25 | 10 | 16 | 26 | 10 | 16 |
QMTFG RLQGISPKI M |
p98-112 | 34 | 8 | 16 | 10 | 22 | 22 |
TFGRLQGISPK |
p100-110 | NA | |||||
FG RLQGISPKI |
p101-111 | NA | |||||
G RLQGISPKI M |
p102-112 | NA | |||||
RLQGISPKI MP |
p103-113 | NA | |||||
LQGISPKIMPK |
p104-114 | NA | |||||
FG RLQGISPKI MPKK |
p101-115 | 29 | 11 | 20 | 22 | 13 | 18 |
RKQLVIYEEISDPEE |
p171-185 | 13 | 18 | 8 | 14 | 6 | 26 |
| Caucasian allele frequency (%) | 8.7 | 10 | 8 | 11.7 | 8.3 | 12.1 | |
Binding scores of the respective peptides predicted by SYFPEITHI are shown. Scores of 20 and more are considered to have a high probability of binding to the respective HLA-DR subtype. The amino acids printed in bold letters represent the sequence of the HLA-A2-restricted epitope p103-111 with a binding score for HLA-A*0201 of 23. “NA” (not available) indicates that SYFPEITHI provides MHC-II predictions only for polypeptides of a length of at least 15 amino acids