Fig. 4.

Primary Bc bind NGcGM3 and binding can be inhibited by anti-CD1d. a Tonsillar (left) and peripheral blood (right) sorted Bc were incubated for 24 hs with empty liposomes (gray line histogram) or with NGcGM3-loaded liposomes (black line histogram), followed by staining with a mAb (14F7, referred as aNGcGM3 in the figure) or isotype-matched control mAb (dashed histogram). The numbers represent the percentages of NGcGM3-binding Bc. Data are representative of three independent experiments. b Upper panels tonsillar (left) and peripheral blood (right) sorted Bc were pretreated for 2 hs at 37 °C with aCD1d mAb (clone CD1d42) prior treatment as in a. The numbers represent the percentages of NGcGM3-binding Bc. Data are representative of three independent experiments. Lower panel: bars show the ratio between the percentage of cells binding NGcGM3 when CD1d is blocked and when it is not (mean of three independent experiments ± SEM)