Fig. 6.

Addition of NGcGM3 to co-cultures of Bc and iNKT induces proliferation that can be inhibited by aCD1d. a Representative flow cytometry dot plots showing expression of CD3 and Ki-67 in co-cultures of Bc and expanded iNKT in the presence of empty liposomes (left, referred as vehicle in the figure), NGcGM3-loaded liposomes (middle) and NGcGM3-loaded liposomes plus blocking aCD1d mAb (right). Numbers indicate the percentages of cells in the quadrants. b Histogram presenting the proportions of CD3⁺Ki-67⁺ cells in co-cultures, as in a, normalized to those of the empty liposomes (referred as vehicle in the figure) supplemented cultures. c Histogram presenting the proportions of CD19⁺Ki-67⁺ cells in co-cultures, as in a, normalized to those of the empty liposome (referred as vehicle in the figure) supplemented cultures. Data in b and c show the mean of the pool ratios ± SEM from four independent experiments. *p < 0.05 and **p < 0.01, unpaired t test