Fig. 5.

MC38 ApoLinkerP144 induces memory immune responses in vivo. C57BL/6 mice were subcutaneously injected with 5 × 10⁵ MC38 ApoLinkerP144 cells/mice. 35 days later, most of the mice remained tumor-free. a An ELISPOT assay was performed with splenocytes of MC38 ApoLinkerP144 tumor-free mice. Results show interferon gamma spots among total splenocytes. Mean ± SEM ***P < 0.001. b 35-day MC38 ApoLinkerP144 tumor-free mice were rechallenged subcutaneously in the right flank with 5 × 10⁵ MC38 parental cells. Survival results are displayed as a Kaplan–Meier plot of tumor occurrence. Treatment groups were compared using the log-rank test. ***P < 0.001