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. 2014 Jun 20;63(10):1023–1036. doi: 10.1007/s00262-014-1571-6

Fig. 1.

Fig. 1

IMiDs exert a costimulatory effect on T cells from MM patients. a Purified CD8+ T cells from MM patients were stimulated with anti-CD3 and anti-CD28-coated microbeads. After 6 days, culture supernatants were harvested and cytokine concentration was determined by ELISA. Each symbol represents one experiment, and the mean of all experiments is indicated by the line. b–e T cells from MM patients were stimulated with anti-CD3 and anti-CD28-coated microbeads. After 3 days of T-cell stimulation, intracellular flow cytometry stainings were performed to evaluate the IFN-γ, TNF-α, IL-2 and MIP-1β production by the CD4+ and CD8+ T-cell compartment. b Flow cytometry plots showing the percentages of IFN-γ+, TNF-α+ and IFN-γ+ TNF-α+ CD8+ and CD4+ T cells of one representative out of 4 experiments. c The bar graphs cytokine production by CD8+ and CD4+ T cells. Data are from 5 independent experiments, represented as mean ± standard error of the mean (SEM). d The pie charts indicate the proportion of polyfunctional T cells (one representative out of five experiments is shown). e The bar graphs show the fold increase of CD8+ T cells and CD4+ T cells exerting either 1 or ≥ 2 of the following functions: IFN-γ, TNF-α, IL-2 and MIP-1β production compared to the ‘no IMiDs’ condition. Data are from 5 independent experiments, represented as mean ± standard error of the mean (SEM). f–g Purified CD8+ T cells from MM patients were stimulated with anti-CD3 and anti-CD28-coated microbeads. After 3 days of T-cell stimulation, intracellular flow cytometry stainings were performed to evaluate the CD107a, IFN-γ and perforin expression by the CD8+ T cells. f Flow cytometry plots showing the percentages of IFN-γ+, CD107a+ and IFN-γ+ CD107a+ CD8+ T cells of one representative out of 3 experiments. g The bar graphs show the percentages of perforin+ CD8+ T cells. Data are from 3 independent experiments, represented as mean ± standard error of the mean (SEM). All statistically significant differences are indicated: ***p < 0.001; **p < 0.01; *p < 0.05