Fig. 3.

Therapeutic vaccination with CAF05 adjuvated vaccine reduces tumor growth. a Mice received an i.v. challenge with 5 × 10⁵ B16-OVA cells and were vaccinated i.p. at day 2, 6, and 10 relative to the day of challenge, photos are the representative of lungs taken 16 days after challenge. b Average + SEM number of lung tumors 16 days after challenge, lungs with too many tumors to count were defined as 300, P value is Kruskal–Wallis nonparametric test, N = 16 (naïve) or N = 8 (vaccine groups). c Mice were challenged intradermally with 5 × 10⁴ TC-1 cells, vaccinated i.p. on day 9, 12, 15, and 29 (arrows) relative to tumor challenge, and tumor sizes was recorded for 54 days; there was a significant (P < 0.05) difference in tumor size between E7 + CAF05-vaccinated and naïve mice from day 18 onwards and between E7 + CAF01-vaccinated mice and naïve on day 25 only, P < 0.05, ANOVA w/Tukey’s posttest (on individual days), N = 8 all groups. The mean tumor size fluctuates at late time point in some groups due to euthanization of mice with tumors >200 mm². d Mice in Fig. 3c were euthanized when a humane end point was met, log-rank test of survival data: saline versus E7 + CAF05 P < 0.0001, E7 + CAF01 versus E7 + CAF05 P = 0.0047, saline versus E7 + CAF01 P = 0.0082. There were some non-humane end point-related deaths during bleeding of mice on day 14, 18, and 25. e TC-1 tumor growth (5 × 10⁴ TC-1 cells given intradermally) of mice vaccinated i.p. on day 11, 15, 18, 25, 41, and 47 (arrows); there was a significant (P < 0.05) difference in tumor size between naïve and E7 + poly(I:C) and naïve and E7 + Mont.ISA720/poly(I:C) from day 18 onwards and between naïve and E7 + CAF05 from day 21 onwards, ANOVA w/Tukey’s posttest (on individual days), N = 7–8. f Mice in Fig. 3e were euthanized when a humane end point was met, log-rank test of survival data: E7 + CAF05 versus naïve or E7 P < 0.0001, E7 + CAF05 versus E7 + Mont.ISA720/poly(I:C) P = 0.0002, E7 + poly(I:C) versus naïve P = 0.0012, E7 + poly(I:C) versus E7 + Mont.ISA720/poly(I:C) P = 0.0032. The remaining four mice in the E7 + Mont.ISA720/poly(I:C) group on day 35 were euthanized due to excessive pain caused by vaccine-induced inflammation of the peritoneum