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The effects of radical therapy on immunosuppressive cells. The gating strategies used to identify a Tregs (CD3+CD4+CD25hiFoxp3+ cells within the lymphocyte gate) and b MDSC (CD14−HLA-DR− and CD15hi within the CD15+ population, to exclude lymphocytes within the monocyte gate). The frequencies of c Tregs and d MDSC were determined pre- and post-treatment. The dotted line indicates the frequency in healthy individuals. Each data set represents n = 16 patients
