Fig. 2.
The characteristics of BM-derived DCs induced by MENK-12 (10−12 mol/L MENK). a, b The expressions of costimulatory molecules on the DCs derived from BM progenitors after treatment with 10−12 mol/L MENK (MENK-12). DCs expressed higher level of MHCII at the concentrations of 10−12 mol/L MENK as well as a significant increase in the number of CD80+ and CD40+ cells on the DC populations. a Dotplot analysis by FCM. b The statistical analysis of histogram by GraphPad prism5. **p < 0.01 versus in the RPIM 1640 group; # p < 0.05 versus in the G+I (GM-CSF plus IL-4) group. c, d IL-12p70 and IL-10 production by BM-derived DCs. Dendritic cells derived from bone marrow progenitors were cultured with GM-CSF plus IL-4 and 10−12 mol/L MENK, respectively, for 7 days. The supernatants were evaluated for the presence of IL-12p70 and IL-10 by ELISA. Results are shown as mean values (±SD) of five independent experiments. **p < 0.01 versus RPIM 1640 group, # p < 0.05 versus G+I group. e–j RT-PCR analysis at mRNA levels of delta, kappa, mu receptors, TNF-α and TLR-9 after treatment with 10–12 mol/L MENK. BM-derived DCs treated with MENK-12 was test group and G+I, RPIM 1640 were control groups. Delta yielded 576 bp. Kappa yielded 609 bp. Mu yielded 218 bp. TNF-α yielded 462 bp and TLR-9 yielded 768 bp. **p < 0.01 versus in the RPIM 1640 group; # p < 0.05 versus in the G+I group; ## p < 0.01 versus in the G+I group