Fig. 1.
Anti-tumor activity induced by L.E.M. extract and/or TRP2 peptide. C57BL/6 mice were injected s.c. in the right foot pad with 7.5 × 105 B16 cells. Beginning the next day, mice were freely fed food containing 2 % L.E.M. extract and received an s.c. injection of CFA containing the TRP2180–188 peptide (200 μg of peptide/mouse). One and 2 weeks after the first immunization, mice received s.c. injections of IFA containing the TRP2180–188 peptide (200 μg of peptide/mouse) for boosting. a Measurement of tumor size. *P < 0.05; **P < 0.01. Ad adjuvant. b On day 21, all mice were euthanized and their feet were amputated. The tumor weights were calculated by subtracting the mean weight of the feet of naïve mice (0.126 g) from the weight of the feet of B16 melanoma-bearing mice. Similar results were obtained in three independent experiments. *P < 0.05; **P < 0.01. NS not significant. c B16 melanoma-bearing mice, which were injected i.p. with either anti-CD8 mAb (200 μg/mouse) or isotype-matched control antibody on days −1 and +7, were treated with or without the combination therapy and tumor size was measured. **P < 0.01. d B16 melanoma-bearing mice were treated with or without L.E.M. extract and Tregs were injected i.v. on days 3 and 7 after tumor inoculation. On day 21, all mice were euthanized, their feet were amputated, and tumor weights were determined. The mean ± SD for each group of 5 or 6 mice is shown. **P < 0.01; NS not significant