Fig. 4.

Significant decrease in the percentage of Tregs in melanoma-bearing mice treated with the combination therapy. B16 melanoma-bearing mice were treated as described in Fig. 1. On day 21, the tumor-draining LNs and spleen were harvested and flow cytometric analysis was performed. a The mean ± SD for each group of 6 mice is shown. Similar results were obtained in three independent experiments. *P < 0.05; **P < 0.01. b Representative results gated on CD4⁺ T cells. Numbers represent the percentage of Foxp3⁺ cells among CD4⁺ T cells. Ad adjuvant