Fig. 5.

Immunohistochemical staining of Foxp3⁺ cells in draining LNs. a B16 melanoma-bearing mice were treated as described in Fig. 1. On day 21, draining LNs were harvested and stained with methyl green. Foxp3⁺ cells in five different fields were counted and the means ± SD are shown. **P < 0.01. b Representative photos. Foxp3⁺ cells are shown as black dots. Ad adjuvant