Fig. 6.

Kinetic analyses of draining LNs and spleen cells of melanoma-bearing mice. B16 melanoma-bearing mice were treated as described in Fig. 1. a On days 0, 7, 14, and 21, the draining LNs and spleen were harvested and stimulated in vitro with each of the indicated peptides in the presence of 20 U/ml IL-2 and the level of IFN-γ in the supernatant was determined using ELISA. The mean ± SD for each group is shown. Similar results were obtained in three independent experiments. *P < 0.05; **P < 0.01. b Flow cytometric analysis for determination of the percentage of Tregs. The mean ± SD for each group of 6 mice is shown. Similar results were obtained in three independent experiments. *P < 0.05; **P < 0.01. Ad adjuvant