Fig. 2.
IL-2-transduced Panc-1 cells induce human NK cell activation and chemotaxis. a 1 × 106 NK cells were stimulated for 5 days with recombinant (rec) IL-2 at 100 IU/mL or conditioned media harvested at day 2 p.i. from 1 × 106 Panc-1 cells buffer-treated (Mock) or infected (MOI = 3 RU/c) with control (∆800), MCP-3 or IL-2-encoding recombinant H-1PV vectors. Stimulated effector NK cells were then co-cultured with NK cell-susceptible Cr51-labelled target K562 cells for 4 h at the indicated effector to target (E/T) cell ratios, and the percentage of specific lysis was calculated as described in the M&M section. Data represent means ± SD of a representative experiment. b 5 × 103 NK cells were placed onto the porous membrane of a 96-well chemotaxis plate and allowed to transmigrate for 3 h into the lower chamber, filled with the conditioned media of (infected) Panc-1 cells, as described in a. NK cells present in the lower chamber were microscopically counted. Data represent the means ± SD of the chemotactic index above buffer-treated cells obtained from three independent experiments