Table 1.
Primers used for cDNA amplification
| Target genea | Primer sequence (5′ → 3′) | TAn. (°C) | Cycles | Amplicon size (bp) |
|---|---|---|---|---|
| m β-actin | catgtttgagaccttcaacacc1 | 58 | 25 | 497 |
| gaaagggtgtaaaacgcagctcagta2 | ||||
| h GAPDH | gccttccgtgtccccactgc1 | 62 | 30 | 334 |
| ggctggtggtccaggggtct2 | ||||
| PV NS1 | ctaaatggaaaggacatcggttggaatag1 | 62 | 30 | 570 |
| gcctccgtcccttggtgg2 | ||||
| PV MCS | tcttgctgcacagcagaggact1 | 63 | 30 | ≥227 |
| gcttcactcacccagttaaccccc2 | ||||
| m NKG2D | tgtggcttgccattttcaaagagacg1 | 58 | 25 | 446 |
| ttacaccgcccttttcatgcagatg2 | ||||
| m Perforin | cagaatgcaagcagaagcacaag1 | 61 | 30 | 486 |
| ggtggagtggaggtttttgtacc2 | ||||
| m Granzyme B | actcaaacacgctacaaga1 | 53 | 30 | 253 |
| atccaggataagaaactcg2 | ||||
| m IFN-γ | tggaggaactggcaaaaggatgg1 | 63 | 30 | 320 |
| cccaccccgaatcagcagcg2 | ||||
| m F4/80 | acaccctcgggctgtgagattgt1 | 58 | 25 | 561 |
| ccccgtctctgtattcaacc2 | ||||
| m iNOS | atggcttgcccctggaagttt1 | 61 | 30 | 827 |
| gcagcatcccctctgatggtg2 | ||||
| m CD11c | ccgtctgagtacccgggcca1 | 61 | 25 | 495 |
| cgctgccactgctggtcctc2 | ||||
| m CD83 | atgtcgcaaggcctccagctc1 | 61 | 25 | 634 |
| tctgatgtgcccttggctttgtaa2 |
T An annealing temperature, bp base pairs, PV parvovirus, GAPDH glyceraldehyde-3-phosphate dehydrogenase, MCS multiple cloning site, iNOS inducible nitric oxide synthase, CD cluster of differentiation
aTarget cDNAs of murine (m) and human (h) origin were amplified with the indicated pair of forward1 and reverse2 primers