Fig. 3.

Spleen-derived Th17 cells express IL-17, IFN-γ and IL-10 and do not express Foxp3. a Schematic experimental procedure. Spleen from naïve C57BL6N and glioma-bearing mice was surgically removed, and splenocytes were co-cultured in vitro with irradiated GL261 tumor cells in the presence or absence of rhTGF-β1. IL-17 secreting cells from different co-culture conditions (see the box below) were isolated and enriched immunomagnetically. Th17 cells were co-injected intracranially (ic) and subcutaneously (sc) with GL261 cells in immune-competent mice. b Th17 cells enriched by immunomagnetic separation were characterized by RT-PCR. All fractions express high levels of IL-17 and different expression of IFN-γ and IL-10 (*P < 0.01; **P < 0.0001; ***P < 0.00005). As shown in histogram, Foxp3 has not been detected