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. 2016 Mar 31;65(6):677–688. doi: 10.1007/s00262-016-1824-7

Fig. 1.

Fig. 1

Overexpression of endostatin in RAW264.7 and BMDM cells alters cell morphology and inhibits cell growth in vitro. a Analysis of BMDM purity by flow cytometry. RAW264.7 and BMDM cells were transfected with pEndostatin plasmid and expression of endostatin mRNA and protein levels were examined by b RT-PCR and c ELISA, respectively. d Light microscopy images of RAW264.7 cells transfected with pEndostatin or pScramble plasmid. e Cell viability of RAW264.7 cells transfected with pEndostatin or control plasmid as determined by MTT assay. Data represent mean ± SD from three independent experiments. *p < 0.05 versus control group; # p < 0.05 versus pScramble group