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. 2014 Mar 20;63(5):501–511. doi: 10.1007/s00262-014-1536-9

Fig. 3.

Fig. 3

Involvement of CD8+ T cells in combined immunotherapy. a CD8+ CD107a+ CTLs are increased in the spleen from mice treated with combination IT relative to naïve or untreated tumor-bearing mice. Overnight stimulation with a mixture of two survivin synthetic epitopes significantly increases the proportion of CD8+ CD107a+ (mean ± SD) in spleen cells from mice receiving combination IT. b Representative dot plots of immunofluorescence analysis for CD107a detection on CD8+ T cells. c The proportion of CD8+ T cells producing IFN-γ (mean ± SD) are increased in mice receiving combination IT, and stimulation with survivin epitopes did not increase the percentage of CD8+IFN-γ+ cells. d Representative dot plots of immunofluorescence analysis for intracellular IFN-γ detection in CD8+ T cells. p values are indicated in panels a and c