Fig. 4.

In vivo role of cytokines and CTLs during combination IT. a Serum IFN-γ, CXCL10 and CXCL9 levels, measured at 19 days after tumor induction, in naive mice, untreated tumor-bearing mice and in mice treated with single agents or combined immunotherapy. Data are shown as mean values + SD in groups of five mice each, *p < 0.03. b Effect of CD8⁺ T cell depletion on combination immunotherapy. In mice depleted of CD8⁺ T cells by an anti-CD8 mAb, combination IT is ineffective as compared to a control group receiving combination IT and an irrelevant IgG mAb (*p = 0.0003 log-rank test). A representative experiment out of two with consistent results is shown