Fig. 6.

Representative ex vivo depletion of isolated leukemia PBMCs. PBMCs were isolated by density gradient centrifugation from the peripheral blood (a–c) or bone marrow (d) of acute or chronic myelomonocytic leukemia patients. The cells were cultivated in the presence of 200 nM hCFPs and controls for 12 h at 37 °C in a 5 % CO₂ atmosphere. The cells were then double-stained with annexin V-eGFP and PI before analysis by flow cytometry. The sum of early-apoptotic and late-apoptotic cells is shown as the mean of duplicates ± SD in comparison with the controls