Fig. 1.

Effect of phloretin on the channel-forming activity of HSP70. Addition of phloretin to the bilayer bathing solution is indicated by an arrow. Planar lipid bilayer was made from an equimolar mixture of DOPS and DOPE. Applied voltage was 100 mV. Bilayer bathing solution was 0.1 M KCl, 5 mM HEPES–KOH (pH 7.0). Concentration of HSP70 in cis-solution was equal to 1.3 mg/ml. Inset HSP70-induced ion channels