Fig. 5.

Phloretin increases immune response caused by HSP70-activating innate immunity. Splenocytes were extracted from spleens of tumor-bearing animals untreated or treated with hydrogels with HSP70 and phloretin separately or in combination 14 days (c) or 21 days (a, b, d) after inoculation. Mice treated with empty hydrogel are indicated as placebo. Sensitivity of naive B16 cells to cytotoxic activity of lymphocytes from mice (a, b) and their ability to induce γIFN release (c, d) were evaluated. Splenocytes from each group were divided into three groups. One was incubated with Dynabeads FlowComp™ Mouse CD8 to remove CD8+ cells (ΔCD8) and the second with Dynabeads FlowComp™ Mouse CD56 (ΔNK) to remove NK cells. The remaining splenocyte population was used as effector cells. The third group was left untreated (total). These lymphocytes were used for CTL assay (b) and for measurement of γIFN (d). * and ** denote a significant difference between treatment groups (p < 0.05 and p < 0.01, respectively)