Fig. 3.

In vitro activation of Hec1A cells by polarized Mϕs. a MTT assays. Result shows the relative number of viable Hec1A cells, which were stimulated for 48 h with CM from polarized Mϕs (Mϕ1/isotype-CM, Mϕ1/αCD40-CM, Mϕ2/isotype-CM or Mϕ2/αCD40-CM). Different superscripts denote significant differences between groups; * p < 0.05, significantly decreased when compared with control mean; ** p < 0.05, significantly increased when compared with control mean. b Invasion assays. Result shows the number of invasive cells/field after a 48 h co-culture period of Hec1A cells with isotype- and CD40-activated Mϕ-1 or Mϕ-2. * p < 0.05, ** p < 0.01, significantly increased when compared to basal level. c RT-PCR analysis. Results show regulation of mRNA expression levels of IL6, LIF, and TNFα in Hec1A cells by soluble factors present in Mϕ1/isotype-CM, Mϕ1/αCD40-CM, Mϕ2/isotype-CM, and Mϕ2/αCD40-CM. The relative mRNA expression of gene of interest (gene/GAPDH ratio) was expressed as fold induction of basal level; ^# p < 0.05, denote significant difference between groups