Fig. 6.

Mϕ-1 induced tumor cell invasion via tumor cell PI3 K/Akt2 signaling pathway activation. a KLE, Hec1A, and EN-1078D cells were stimulated with control media (basal) or CM from Mϕ1/isotype or Mϕ1/αCD40 macrophages for 15 min. Result from Western blot analysis shows representative expression of phosphorylated/active form of Akt (pAkt) and total Akt, from three independent experiments. b KLE cells were transfected with plasmids expressing scramble shRNA (empty) or shRNA against Akt1, Akt2, and Akt3 mRNA. Result shows the number of invasive cells/field after a 48-h co-culture period. * p < 0.01 denotes significant difference between groups. c Control KLE cells (shEmpty) and KLE deficient for Akt1 (shAKt1), Akt2 (shAKt2), and Akt3 (shAkt3) were stimulated with control media (basal) or CM from Mϕ1/isotype or Mϕ1/αCD40 macrophages for 15 min. Result from Western blot analysis shows representative expression of phosphorylated/active form of Akt (pAkt) and total Akt from three independent experiments