Fig. 1.

Eps8-derived peptide-specific T cells from PBMCs secrete IFN-γ. a PBMCs from an HLA-A2.1-positive healthy donor were primed with Eps8-derived peptides and assessed for functional T cells by IFN-γ ELISPOT. T2 cells pulsed with each peptide were used as stimulators. The number of IFN-γ spots in response to modified peptides was statistically significant (P < 0.001) compared to their native counterparts. b IFN-γ ELISPOT assays performed on peptide-primed PBMCs from multiple individuals (n = 6). Experiments performed in triplicate. Data are represented as the mean ± S.D. ^*** P < 0.001, ^* P < 0.05, compared to the native counterpart