Table 1.
Ligand | Ganglidiomab KD (nM)a | 1A7 KD (nM)a |
---|---|---|
14G2a | 53.5 ± 19.20 | 5.35 ± 4.72 |
ch14.18 | 13.1 ± 4.23 | 3.65 ± 2.39 |
ch14.18-IL2 | 49.3 ± 23.40 | 1.13 ± 1.06 |
ch14.18-delta-CH2 | 23.3 ± 10.20 | 10.9 ± 10.30 |
hu14.18 | 10.8 ± 5.01 | 11.8 ± 11.70 |
hu14.18-IL2 | 13.7 ± 4.95 | 10.8 ± 4.97 |
aDissociation constants (KD) of ligands 14G2a, ch14.18/CHO, ch14.18-IL2, ch14.18-delta-CH2, hu14.18, and hu14.18-IL2 from ganglidiomab and 1A7 were determined by surface plasmon resonance in a Biacore X100 system followed by steady-state analysis. Values are means ± SE of 3 independent experiments