Fig. 3.

Gene array analysis reveals NF-κB gene targets associated with macrophage differentiation and high inhibitory potential of EGCG. a Serum-starved HL-60 cells were treated with 3 nM PMA for 18 h. Total RNA was isolated from vehicle-treated cells that remained in suspension and from PMA-treated cells that adhered to the flasks (macrophage-differentiated cells). a Histogram representation of the 89 NF-κB gene targets array expression levels. b Serum-starved HL-60 cells were treated with either 30 μM EGCG or a combination of 3 nM PMA and 30 μM EGCG for 18 h. Total RNA was isolated from EGCG-treated cells that remained in suspension and from PMA-treated cells that adhered to the flasks (macrophage-differentiated cells). Histogram representation of the gene expression array levels represents the percent of gene inhibition