Table 1.
PMA triggers transcriptional increase in NF-κB gene targets in adherent HL-60 cells
| Gene name | Gene ID | Induction by PMA (x-fold) | Functional grouping |
|---|---|---|---|
| IL1RN | 3557 | 556 | Cytokines/chemokines |
| CCL5 | 6352 | 352 | Differentiation, cytokines/chemokines |
| IL1B | 3553 | 200 | Inflammation, cytokines/chemokines |
| ICAM1 | 3383 | 176 | Immune response |
| MMP9 | 4318 | 137 | Differentiation, apoptosis |
| IL1A | 3552 | 102 | Inflammation, cytokines/chemokines |
| IL2RA | 3559 | 71 | Inflammation |
| INS | 3630 | 40 | Inflammation, apoptosis |
| CD40 | 958 | 27 | Immune response |
| IL8 | 3576 | 24 | Cytokines/chemokines |
| F3 | 2152 | 23 | Anti-apoptosis |
| PDGFB | 5155 | 22 | Stress response |
| TNSF10 | 8743 | 20 | Cytokines/chemokines |
| CD83 | 9308 | 15 | Immune response |
| CCL2 | 6347 | 14 | Immune response, cytokines/chemokines |
| MITF | 4286 | 12 | Differentiation, apoptosis |
| BIRC3 | 330 | 11 | Apoptosis |
| COX2 | 5743 | 10 | Inflammation, apoptosis |
| TNFRSF1B | 7133 | 9.7 | Apoptosis |
Serum-starved HL-60 cells were treated with 3 nM PMA for 18 h. Total RNA was isolated from vehicle-treated cells that remained in suspension and from PMA-treated cells that adhered to the flasks (macrophage-differentiated cells). The identity of only those genes that were induced by tenfold in adherent versus suspension cells is shown and is extracted from Fig. 2a. Data are representative from two independent arrays. Italicized data were further confirmed at the protein and/or activity level