Fig. 2.

Construction and characterization of different CAR-T cells. a A schematic representation of the lentiviral vectors expressing GZ or A28BB is shown. b The expression of GZ and A28BB on the surface of T cells was demonstrated by assessing the expression of eGFP and mCherry, respectively. c The flow cytometric analysis of the CD4⁺ and CD8⁺ phenotypes of in vitro expanded T cells. d Fourteen days after transduction, the expression levels of CD45RO and CD62L were determined using a FACSCalibur with the indicated antibodies. e The results were consistent in 3 donors