Fig. 5.

Significant suppression of tumor growth in vivo by dual-targeted T cells requires both GPC3 and ASGR1 expression in the tumor cells. GZ+A28BB T cells were infused intravenously into NOD/SCID mice bearing established GPC3⁺ or/and ASGR1⁺MHCC-97L tumor xenografts. The tumor volumes (a) and weights (b) were quantified. c Formalin-fixed, paraffin-embedded tumor and mouse liver sections were consecutively cut and stained for human CD3e to detect T cell infiltration (brown). The images were taken with a microscope (Axio Scope.A1, Zeiss) under × 200 magnification. The scale bar was 100 μm. d The levels of IFN-γ, IL-2, TNF-α and IL-4 in mouse serum were evaluated by an ELISA