Fig. 1.

Th3 population is augmented in PBL from untreated melanoma patients compared with normal donors and decreased in DTH⁺ but not in DTH⁻ patients after DCs vaccination. PBMC from 14 healthy donors (Healthy) were stained with anti-CD4 and anti-LAP-TGF-β antibodies and analyzed by flow cytometry. The obtained results were compared with Th3 proportion from 14 DTH⁺ patients and 8 DTH⁻ patients before (untreated) and after a complete immunotherapy cycle (treated). Th3 percentage on healthy donors compared with melanoma untreated or treated patients. a Comparison of expression of Th3 percentage in PBMC between healthy donors, DTH⁻ and DTH⁺ patients before (untreated) and after immunotherapy (treated). b Detection of free TGFβ in serum of DTH⁻ and DTH⁺ patients was performed by ELISA assay. Statistical analyzes were performed using one-way ANOVA and unpaired Student’s t-test (**p < 0.01, ***p < 0.001)