Fig. 2.

CD4⁺ IFNγ⁺ T lymphocyte population is more potently induced after DCs vaccination in DTH⁺ than in DTH⁻ melanoma patients. PBMC from DTH⁺ and DTH⁻ patients before (untreated) and after (treated) a complete immunotherapy cycle were activated with PMA/Ionomicyne, incubated with antibodies against CD4 and IFNγ and analyzed by flow cytometry. a Panel displaying density plots from one representative analysis of Th1 profile (upper). Th1 percentage based on CD4⁺ IFNγ⁺ T cells in DTH⁺ (untreated and treated, n = 8) and DTH⁻ (untreated and treated, n = 8) patients. b An ELISA assay showing the IFNγ secretion in DTH⁻ and DTH⁺ patients. Statistical analyzes were performed using paired Student’s t-test (*p < 0.05)