Fig. 4.

Proliferation assay of splenocytes. CEA-expressing CEA2682 mice were immunized with uP-PS/scFv6.C4 or uP, as described in the legend of Fig. 3 and Materials and Methods section. Fifteen days later, these mice were euthanized to obtain splenocytes, which were labeled with 2.5 µM CSFE and stimulated with CEA or scFv6.C4 for 6 days. Frequency of CD4+ (a), CD8+ (b) and CD45R/B220 (c) CFSE-low cells were determined by flow cytometry. Data are expressed as mean ± SD of each group of mice (4 per group). Representative data of 2 biological replicate experiments are shown. *p ≤ 0.05, **p ≤ 0.01