Fig. 5.

N-3 PUFA treatment inhibits IFN-γR expression and IFN-γ signaling in DU-145 cells. a Western blot analysis of IFN-γRα expression in DU-145 cells pre-treated with 100 μM PUFA for 24 h. b–f Western blot measurement of pho-JAK1 and JAK1 (b), pho-STAT1 and STAT1 (c), pho-ERK1/2 and ERK1/2 (d), pho-JNK and JNK (e), and pho-P38 and P38 (f) in DU-145 cells pre-treated with 100 μM PUFA for 24 h, followed with 10 ng/mL IFN-γ treatment for 30 min. Data are representative of three independent experiments with similar result. *p < 0.05; **p < 0.01; ***p < 0.001, compared with untreated cells (a) or cells treated with IFN-γ alone (b–f), one-way ANOVA followed with Bonferroni posttest. Error bars represent SEM (n = 3). Un untreated