Fig. 2.
Increased levels of serum IL-6 and TNF-α in naïve aged mice. a Serum levels of IL-6 and TNF-α in naïve and CT26-bearing (on day 10) young (7 W) and aged (60 W) BALB/c mice were measured by ELISA. Mice were injected s.c. with 1 × 105 CT26 cells. Each group consisted of five or six mice. *P < 0.05, **P < 0.01. Similar results were obtained in two independent experiments. b The spleen cells from three young (7 W) and three aged (60 W) BALB/c mice were examined for their production of IL-6 and TNF-α during two days in culture in the presence or absence of LPS (5 μg/mL) or anti-CD3 mAb (2 μg/mL). **P < 0.01. NS not significant. Similar results were obtained in two independent experiments. c Nude young (7 W) and aged (45 W) mice were injected s.c. with 1 × 105 CT26 cells, and tumor growth was measured. Each group consisted of five mice. d Serum levels of IL-6 and TNF-α in naïve and CT26-bearing (on day 18) young (7 W) and aged (45 W) nude mice were measured by ELISA. Each group consisted of five mice. *P < 0.05