FIG. 5.

Northern blot analysis of RNA extracted from Δγ381 particles and wt FHV particles. RNA (100 ng of Δγ381 RNA per lane; 5 ng of wt RNA per lane) was size fractionated on a 1% agarose-formaldehyde gel and transferred to a nylon membrane. Following UV cross-linking, the immobilized RNAs were hybridized to digoxigenin-UTP-labeled negative-sense RNA probes complementary to three different regions of RNA2 or RNA1. Hybridization complexes were visualized by chemiluminescence. (A) Hybridization with probes complementary to RNA2. Probe I was complementary to nt 1 to 488, probe II was complementary to nt 600 to 888, and probe III was complementary to nt 1000 to 1400. Note that full-length RNA in the Δγ381 sample migrates slightly faster than full-length wt RNA2 due to a deletion of 78 nt. (B) Hybridization with probes complementary to RNA1. Probe I was complementary to nt 1 to 980, probe II was complementary to nt 1000 to 1994, and probe III was complementary to nt 1998 to 3107. Note that samples hybridized with probe I migrated at a slight angle during gel electrophoresis, explaining the apparently larger molecular size of RNA1 in the Δγ381 sample than in the wt sample.