FIG. 6.

Electrophoretic analysis of total cellular RNA isolated from Drosophila cells transfected either with wt FHV RNAs or RNAs purified from Δγ381 particles. Drosophila cell monolayers (10⁷ cells) were transfected either with 100 ng of wt RNA1 and 100 ng of capped in vitro-synthesized wt RNA2 or with approximately 1.5 μg of total RNA purified from Δγ381 particles. After 24 h, total cellular RNA was purified from the transfected cells and an aliquot of 3 μg was size fractionated on a 1% agarose-formaldehyde gel. RNAs were visualized with ethidium bromide. Lane 1, RNA molecular size markers; lane 2, total RNA from mock-transfected cells; lane 3, total RNA from cells transfected with wt FHV RNAs; lane 4, total RNA from cells transfected with RNA purified from Δγ381 particles. Note that RNA2 in this lane migrated slightly faster than wt RNA2 in lane 3 due to a deletion of 78 nt. The major band between RNA1 and RNA2 represents the two rRNAs which did not resolve under the electrophoresis conditions used here.