TABLE 1.

Properties of soluble and assembled mutant and wt coat precursor proteins

Mutant or wt coat protein alpha	% Efficiency of assemblya	Cleavage of alpha protein in assembled particles	Specific encapsidation of FHV RNA1 and RNA2	% Infectivity of assembled virionsb
Δγ363	NAc	NA	NA	NA
Δγ381	30	Normal	+	<(1.5 × 10−6)d
Δγ387	30	Normal	+	<(1.5 × 10−6)d
Δγ394	30	Normal	+	<(1.5 × 10−6)d
Δγ400	30	Normal	+	<(1.5 × 10−6)d
Δγ403	100	Normal	++	11
Δγ405	100	Normal	+++	44
F402A	100	Normal	+++	8
E403A	100	Normal	++++	55
F405A	100	Normal	+++	35
F407A	100	Normal	+++	27
wt	100	Normal	++++	100

^aRelative to wt protein. 

^bRelative to specific infectivity of a reference preparation of gradient-purified wt FHV (10.7 × 10¹⁰ PFU/mg). 

^cNA, not applicable. 

^dNo plaques detected.