Fig. 4.
CAR-RNA-transfected T cells prolong survival of mice after co-injection with MCSP+ tumor cells. T cells from healthy donors were electroporated with RNA encoding a CEA-specific (CEA CD28-CD3ζ) or MCSP-specific (MCSPHL CD28-CD3ζ) CAR, or without RNA. Cells were cryoconserved 2 h after electroporation. Thawed T cells were co-injected with MCSP+ Melur cells subcutaneously into Rag−/− cγ−/− mice, and their survival was determined. Data are presented in a Kaplan–Meier plot. Each group consisted of seven animals. MST mean survival time. The p values, determined by a Mann–Whitney U test, were: no RNA versus CEA CD28-CD3ζ, 0.81; no RNA versus MCSPHL CD28-CD3ζ, 0.0006; and CEA CD28-CD3ζ versus MCSPHL CD28-CD3ζ, 0.007