Fig. 3.

Downregulation of ADAM17. Breast carcinoma cells (MDA-MB-231) were treated for 1 h with mAb A300E (a) or mAb E20-19 (b) at 4 °C. After washing, cells were incubated for designated times either at 37 °C (filled square) or at 4 °C (filled circle). Cell surface-bound antibodies were detected by FACS analysis using an allophycocyanin-conjugated anti-mouse antibody. % mAb remaining on cell surface corresponding to 0 h incubation time was set arbitrary to 100 %