Fig. 3.

Association between HLA-A*02:01 and HCMV. a Detection of HCMV UL55 gene in the peripheral blood of glioma patients and control subjects by nested PCR. Grade II, Grade III, and Grade IV are different grades of glioma. b HCMV DNA was more frequently detected in the peripheral blood of HLA-A*02:01-negative than -positive glioma patients (36.5 vs. 8.3%, P = 0.007). c Representative micrographs from glioma cases showing IE1-72 and pp65 positivity (G1, G2, and G3) and negativity (G4). d, e Expression of HCMV proteins IE1-72 (d, 88.1 vs. 25%, P < 0.001) and pp65 (e, 73.8 vs. 25%, P < 0.001) was more frequently detected in tumor tissue of HLA-A*02:01-negative than -positive glioma patients. f Anti-HCMV IgM was more frequently detected in the serum of HLA-A*0201-negative than -positive glioma patients (21.4 vs. 4.2%, P = 0.047). g–i Prognostic value of HLA-A*02:01 allele and presence of HCMV DNA in 95 GBM patients, as determined by Kaplan–Meier analyses. Neither HLA-A*02:01 (g) nor HCMV (h) was associated with OS. The status of HCMV in conjunction with HLA-A*02:01 was also not correlated with GBM patient survival (i)