Fig. 3.

Soluble MICA production of IL-1β-treated various cancer cells. Various cancer cells including MKN1, HCT116, HT29 and HeLa cells were treated in the presence or absence of IL-1β (50 ng/ml) for 48 h. Soluble MICA production of IL-1β-treated or non-treated various cancer cells was evaluated by specific ELISA (right panel). sMICA, soluble MICA. We also examined the expression of membrane-bound MICA on IL-1β-treated or non-treated various cancer cells by flow cytometry (left panel). Black line histograms, MICA staining of non-treated cells; Gray line histograms, MICA staining of IL-1β-treated cells; shaded/black histograms, control IgG isotype Ab staining. Similar results were obtained from two independent experiments. *p < 0.05