Fig. 5.

Effect of various doses of paclitaxel on T cells in vitro and in vivo. To evaluate the effects of various doses of paclitaxel on lymphocyte proliferation, spleen cells from naive C57BL/c mice were treated in quadruplicate with different concentrations of paclitaxel in presence of 2 µg/ml ConA for 3 days in 96-well flat-bottom tissue culture plates, proliferation responses were evaluated by MTT (a). Spleen cells were prepared from naive mice treated with paclitaxel at dose levels of 0, 4, 20 and 40 mg/kg for 2 days, and seeded into 96-well flat-bottom tissue culture plates containing 2 µg/ml ConA complete medium, proliferation responses were evaluated by MTT as above (b). Optical density (OD) at 570 nm was calculated as mean ± SE. To evaluate the effects of various doses of paclitaxel on spleen cells and T-cell subpopulations, naive C57BL/c mice were treated with various doses of paclitaxel, the total number of spleen cells were calculated from on day 0 to on day 7 after paclitaxel injection (c). T-cell subpopulations of spleen were assayed 2 days after a single treatment with various doses of paclitaxel (d). Typical dada from a representative experiment for D (n = 5) were presented (e). *P < 0.05 and **P < 0.01 compared with non-treatment control group