Fig. 6.

The effect of various concentrations of paclitaxel on dendritic cell in vitro. To evaluate the effect of various concentrations paclitaxel on DCs, the cultured-7 days dendritic cells were exploded in various concentrations of paclitaxel for 3 days and the proportion of apoptosis cells were assayed by FACS (a). To evaluate the effect of 20 nM paclitaxel on the maturation of DCs in presence of tumor cell (or tumor cell cultured supernatant) or not, after bone marrow-derived dendritic cell precursors (0.5 × 106/mL) were cultured for 4 days, RM-1 tumor-cell (0.5 × 104/mL) or RM-1 cell (0.5 × 106/mL) cultured supernatant combination with or without 20 nM paclitaxel were added to dendritic cell cultures (b). Dendritic cells were harvested on day 7, and the proportion of DC maturation was assayed by FACS (n = 3)