Fig. 7.

Effects on DC activation in vivo. Mice were treated with decitabine (DAC) and/or ODN1826 (ODN) for 1 week. A group of mice was not treated (NT). Regional lymph nodes were then collected. a CD11c⁺DC-, CD11c⁺BB20⁺ pDC-, CD11c⁺CD11b⁺ mDC- and CD11c⁺CD86⁺-activated DC (aDC) phenotypes were determined in lymph node by flow cytometry. Data represent mean cell numbers ± SD, n = 4 mice per group. *P < 0.05 compared to NT; **P < 0.05 compared to without DAC. b CD11c⁺ DC were isolated from lymph nodes by MACS and IL-12 and IL-10 mRNA expression was assessed by qRT-PCR. Data represent the mean IL-12/IL-10 ratio, n = 4 mice per group