Fig. 5.

NKG2D expression and cytotoxic activity of 5 days (d) IL15-stimulated NK cells are not inhibited by sarcoma cells. a After the IL15-stimulated 40 h coculture (second bar), TCD PBMC were removed from STA-ET2.1 cells, as indicated by the arrows, and subsequently incubated with IL15 for three additional days in the presence (fourth bar) or absence of fresh STA-ET2.1 cells (fifth bar). NKG2D expression on NK cells was analyzed after the 40 h coculture and after the 40 h plus 3 days (d) coculture. Data are combined from multiple experiments. b Purified NK cells were activated with IL15 for 5 days and afterward cocultured with sarcoma cells for 40 h (gray bars, IL15 stimulated samples). Data of NKG2D expression are combined from multiple experiments. c Lysis of non-cocultured STA-ET2.1 and HOS cells after the coculture of 5 days IL15-activated NK cells with sarcoma cells. Data are analyzed at a 10:1 E:T ratio and combined from three to two experiments, respectively